• Bioprospecting for Thermozymes and Characterization of a Novel Lipolytic Thermozyme Belonging to the SGNH/GDSL Family of Hydrolases 

      Escuder-Rodríguez, Juan-José; De Castro de Antonio, María Eugenia; Saavedra-Bouza, Almudena; González-Siso, María-Isabel; Becerra, Manuel (MDPI, 2022-05-20)
      [Abstract] Functional screenings were conducted on two metagenomic libraries from hot springs in order to find novel thermozymes with potential biotechnological applications. These included enzymes acting on plant cell ...
    • Cellulases from thermophiles found by metagenomics 

      Escuder-Rodríguez, Juan-José; De Castro de Antonio, María Eugenia; Cerdán, María Esperanza; Rodríguez-Belmonte, Esther; Becerra, Manuel; González-Siso, María-Isabel (MDPI, 2018-07-10)
      [Abstract] Cellulases are a heterogeneous group of enzymes that synergistically catalyze the hydrolysis of cellulose, the major component of plant biomass. Such reaction has biotechnological applications in a broad spectrum ...
    • Comparative Metagenomic Analysis of Two Hot Springs From Ourense (Northwestern Spain) and Others Worldwide 

      De Castro de Antonio, María Eugenia; Escuder-Rodríguez, Juan-José; Becerra, Manuel; Rodríguez-Belmonte, Esther; González-Siso, María-Isabel (Frontiers, 2021-11-26)
      [Abstract] With their circumneutral pH and their moderate temperature (66 and 68°C, respectively), As Burgas and Muiño da Veiga are two important human-use hot springs, previously studied with traditional culture methods, ...
    • Exploring the Taxonomical and Functional Profile of As Burgas Hot Spring Focusing On Thermostable β-galactosidases 

      De Castro de Antonio, María Eugenia; Doane, Michael P.; Dinsdale, Elizabeth Ann; Rodríguez-Belmonte, Esther; González-Siso, María-Isabel (Springer Nature, 2021-01-08)
      [Abstract] In the present study we investigate the microbial community inhabiting As Burgas geothermal spring, located in Ourense (Galicia, Spain). The approximately 23 Gbp of Illumina sequences generated for each replicate ...
    • Insights on Microbial Communities Inhabiting Non-Volcanic Hot Springs 

      Escuder-Rodríguez, Juan-José; De Castro de Antonio, María Eugenia; Saavedra-Bouza, Almudena; Becerra, Manuel; González-Siso, María-Isabel (MDPI, 2022-10-13)
      [Abstract] The northwest of Spain has an abundance of non-volcanic hot springs that, until recently, had only been used for thermalism activities. One of such hot springs, Muiño da Veiga, has now been explored using ...
    • Searching for Novel Thermostable β-galactosidases 

      De Castro de Antonio, María Eugenia (2021)
      [Abstract] β-galactosidases are biotechnologically interesting enzymes for the hydrolysis of lactose and GOS synthesis. The main objective of this work was to analyze the microbial community structure and the functional ...
    • Xylanases From Thermophilic Archaea: A Hidden Treasure 

      Saavedra-Bouza, Almudena; Escuder-Rodríguez, Juan-José; De Castro de Antonio, María Eugenia; Becerra, Manuel; González-Siso, María-Isabel (Elsevier, 2022-12-12)
      [Abstract] Archaea are a domain of prokaryotic organisms that often inhabit extremophilic environments. Their enzymes are therefore of enormous biotechnological interest, due to their stability and activity in harsh ...